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In vitro bioassay for type E prostaglandins based on their NK immunodepressing activity
T E Kluchareva1, V A Matveeva, N E Kushlinsky
1All-Union Cancer Research Center, Moscow, Russia.
Immunology Letters
|August 1, 1992
Abstract:
A biological assay for PGE in commercial preparations, or secreted by tumor cells in culture fluid was developed on the basis of the immunosuppressive effect of PGE on the cytotoxic activity of NK cells. The assay is simple, rapid and convenient for detecting PGE cell secretion, either spontaneous or induced by various signals. The sensitivity of the bioassay is limited by the sensitivity of NK cells to the immunosuppressive activity of PGE (i.e., about 10(-8) M).