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Related Experiment Videos

A comparative study of blood lactate analytic methods.

F A Rodríguez1, M Banquells, V Pons

  • 1Centre d'Alt Rendiment (CAR), Departament de Fisiologia i Valoració Funcional, Barcelona, Spain.

International Journal of Sports Medicine
|August 1, 1992
PubMed
Summary

Comparing blood lactate methods, electroenzymatic (EE) assays consistently yielded lower results than photoenzymatic (PHE) assays. These differences, partly due to measuring different lactate fractions, can be mitigated with sample additives.

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Area of Science:

  • Clinical Chemistry
  • Biomedical Analysis

Background:

  • Accurate blood lactate measurement is crucial for diagnosing and managing various medical conditions, including critical illnesses and athletic performance.
  • Different analytical methods exist for blood lactate determination, each with potential variations in results.

Purpose of the Study:

  • To compare the precision, accuracy, linearity, and intermethod agreement of three electroenzymatic (EE) semiautomatic assays against a photoenzymatic (PHE) assay for blood lactate analysis.
  • To investigate the factors contributing to discrepancies between these methods and explore potential solutions for harmonization.

Main Methods:

  • Evaluation of three electroenzymatic (EE1, EE2, EE3) and one photoenzymatic (PHE) blood lactate analytic methods.
  • Testing involved reference standards and duplicate capillary blood samples, assessing precision, accuracy, and linearity across the measurement range.

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  • Intermethod comparison was performed, with and without sample pre-treatment using a hemolyser and glycolytic inhibitor.

    • Main Results:

    • All tested methods demonstrated good precision and accuracy for L-lactate standards (VC 1.78-3.38%, difference 1.81-3.38%).
    • High correlation (r=0.913-0.946) was observed between methods, but EE assays systematically underestimated lactate levels compared to PHE.
    • Differences between EE and PHE methods were concentration-dependent and significantly reduced by adding a hemolyser and glycolytic inhibitor, suggesting intraerythrocytic lactate and glycolysis as contributing factors.

    Conclusions:

    • The electroenzymatic and photoenzymatic blood lactate assays are not interchangeable for capillary blood samples due to systematic differences.
    • The PHE method measures total blood lactate, while EE methods primarily measure plasmatic-extraerythrocytic lactate.
    • Regression equations are provided to facilitate conversion between PHE and EE assay results, aiding in data harmonization.