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M13 DNA fingerprinting, a new tool for classification and identification of Lactobacillus spp
V I Miteva1, A N Abadjieva, T T Stefanova
1Institute of Microbiology, Bulgarian Academy of Sciences, Sofia.
The Journal of Applied Bacteriology
|October 1, 1992
Abstract:
The optimal conditions for the application of M13 DNA fingerprinting to the genus Lactobacillus were determined. Comparative fingerprint analysis of representative strains of Lactobacillus delbrueckii subsp. delbrueckii, Lact. delbrueckii subsp. lactis, Lact. delbrueckii subsp. bulgaricus, Lact. helveticus and Lact. casei permitted the differentiation of species, subspecies and individual strains and the quantitative determination of their genetic relatedness. The results confirm the high specificity of M13 DNA fingerprinting and indicate that it might be used in the classification of Lactobacillus spp.