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A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
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An unexpected complication following immunoadsorption with a staphylococcal protein A column

D W Huestis1, R M Rifkin, B G Durie

  • 1Department of Pathology, University of Arizona College of Medicine, Tucson 85724.

Journal of Clinical Apheresis
|January 1, 1992
PubMed

Extracorporeal immune adsorption with staphylococcal protein A (SPA) columns can remove immune complexes and immunoglobulins in the treatment of a variety of diseases. We present the case of an elderly man with neuropathy associated with monoclonal gammopathy, treated by 3 on-line SPA procedures. At the completion of these treatments his neuropathy relapsed, progressing to near-total paralysis. Return to a baseline clinical status required several months. The reason for this severe relapse is not clear. Possible explanations include SPA activation of T-lymphocytes, with release of gamma interferon and increased antigen recognition, or removal of an antiidiotype control mechanism. We advise caution in the application of immunoadsorption to conditions in which it has not yet been evaluated.

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