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Related Experiment Videos

Sugar uptake into brush border vesicles from normal human kidney.

R J Turner, M Silverman

    Proceedings of the National Academy of Sciences of the United States of America
    |July 1, 1977
    PubMed
    Summary
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    Human kidney cortex vesicles show sodium-dependent D-glucose uptake, suggesting two transporters in the proximal tubule brush border. This research aids understanding of renal glucose transport mechanisms.

    Area of Science:

    • Nephrology
    • Molecular Biology
    • Biochemistry

    Background:

    • The proximal tubule is crucial for reabsorbing filtered glucose.
    • Understanding glucose transport mechanisms is vital for kidney function and metabolic disease research.

    Purpose of the Study:

    • To characterize the sodium-dependent D-glucose transport system in human kidney cortex.
    • To identify the localization and kinetic properties of glucose transporters.

    Main Methods:

    • Preparation of osmotically active vesicles from normal human kidney cortex.
    • Uptake studies of simple sugars, focusing on D-glucose.
    • Analysis of sodium-dependency, phlorizin-sensitivity, and substrate specificity.
    • Kinetic analysis using Scatchard plots to determine transporter characteristics.

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    Main Results:

    • D-glucose uptake was sodium-dependent and phlorizin-sensitive.
    • Transport specificity included alpha-methyl-D-glucoside and D-galactose, but not D-fructose or D-mannose.
    • Scatchard analysis indicated two sodium-dependent D-glucose transporters with distinct affinities (Km ≈ 0.3 mM and Km ≈ 6 mM).

    Conclusions:

    • The characterized sodium-dependent D-glucose transport is likely located in the brush border of the proximal tubule.
    • Evidence suggests the presence of at least two distinct D-glucose transporters in this location.
    • These findings contribute to understanding renal glucose handling and potential therapeutic targets.