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Modified nucleosides in human serum.

E P Mitchell1, L Evans, P Schultz

  • 1Department of Medicine, University of Missouri-Columbia 65201.

Journal of Chromatography
|October 2, 1992
PubMed
Summary
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Modified nucleosides in serum, measured using advanced chromatography, show potential as cancer biomarkers. Elevated levels in cancer patients, regardless of age or sex, highlight their diagnostic value.

Area of Science:

  • Biochemistry
  • Oncology
  • Analytical Chemistry

Background:

  • Methylated purines and pyrimidines from transfer ribonucleic acid degradation are excreted abnormally in cancer patients.
  • Modified nucleosides are potential indicators of cancer.

Purpose of the Study:

  • Establish normal serum levels of ten modified nucleosides in healthy adults.
  • Correlate modified nucleoside levels with age and sex.
  • Determine if serum levels differ in patients with malignancies.

Main Methods:

  • Utilized reversed-phase high-performance liquid chromatography with diode-array measurement.
  • Quantified ten modified nucleosides in serum samples.
  • Analyzed serum from 37 healthy adults and patients with various malignancies.

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Main Results:

  • Normal serum nucleoside levels were reproducible and showed no significant variation by age or sex.
  • Patients with malignancies exhibited consistently elevated serum nucleoside levels.
  • Elevated levels were highest in patients with advanced-stage cancer.

Conclusions:

  • Modified nucleosides in serum are reliable biomarkers for cancer detection.
  • Serum nucleoside levels are independent of age and sex in healthy individuals.
  • Increased serum nucleoside levels correlate with cancer presence and disease progression.