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Capillary electrophoretic determination of the protease Savinase in cultivation broth
A Vinther1, J Petersen, H Søeberg
1Novo Nordisk A/S, Bagsvaerd, Denmark.
Journal of Chromatography
|September 11, 1992
Abstract:
The highly basic washing enzyme Savinase and various analogues were analysed by micellar electrokinetic chromatography (MEKC) and electrophoresis. Broth samples were withdrawn during the cultivation of Savinase by recombinant microorganisms. Savinase peak areas obtained by MEKC-electrophoretic analysis were normalized with respect to migration time and compared with traditional enzyme activity measurements. The electropherograms indicated thermal degradation of the Savinase molecule at high field strengths. Baseline separation of Savinase and two analogues was achieved.