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Optimized Protocol for Retinal Wholemount Preparation for Imaging and Immunohistochemistry
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A new acrylic resin formulation: a useful tool for histological, ultrastructural, and immunocytochemical

C Scala1, G Cenacchi, C Ferrari

  • 1Istituto di Microscopia Elettronica Clinica, Policlinico S. Orsola, Università di Bologna, Italy.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|November 1, 1992
PubMed

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...