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EFFECT OF MUKERJEE'S GROUP IV PHAGE ON EL TOR VIBRIOS

K A MONSUR, S S RIZVI, M I HUQ

    Bulletin of the World Health Organization
    |January 1, 1965
    PubMed

    Insights

    Group IV phage can lyse El Tor vibrios, despite initial resistance. This finding aids in differentiating true cholera from El Tor vibrios, crucial for disease control.

    Area of Science:

    • Microbiology
    • Virology
    • Bacteriology

    Background:

    • El Tor vibrios are typically resistant to Mukerjee's group IV phage at standard concentrations.
    • Accurate differentiation between Vibrio cholerae serovars is critical for epidemiological surveillance and public health interventions.

    Purpose of the Study:

    • To investigate the interaction between group IV phage and El Tor vibrios.
    • To determine if group IV phage can induce lysis in El Tor vibrios under specific conditions.
    • To evaluate the utility of group IV phage as a tool for differentiating El Tor vibrios from other Vibrio cholerae strains.

    Main Methods:

    • Culturing El Tor vibrios and group IV phage.
    • Performing plaque assays and observing lysis on bacterial lawns.
    • Monitoring viable bacterial and phage counts over time.
    • Utilizing darkfield microscopy to assess bacterial motility.
    • Measuring optical density to quantify bacterial lysis.

    Main Results:

    • Sufficient titers of group IV phage can induce clearing on El Tor vibrio lawns, indicative of lysis.
    • A decrease in viable counts of both bacteria and phage was observed when mixed.
    • El Tor vibrios lost motility, and optical density measurements confirmed bacterial lysis.
    • No evidence of group IV phage multiplication within the tested El Tor vibrios was found.

    Conclusions:

    • Group IV phage demonstrates lytic activity against El Tor vibrios, contrary to typical observations at routine dilutions.
    • The observed lysis and lack of phage multiplication suggest a specific interaction rather than a typical lytic cycle.
    • Group IV phage's distinct behavior towards El Tor vibrios makes it a valuable diagnostic marker for distinguishing true cholera strains.
    Keywords:
    BACTERIOPHAGECHOLERAVIBRIO

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