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Related Experiment Videos

Chicken antibodies: a tool to avoid interference by complement activation in ELISA.

A Larsson1, P E Wejåker, P O Forsberg

  • 1Department of Clinical Chemistry, University Hospital, Uppsala, Sweden.

Journal of Immunological Methods
|November 25, 1992
PubMed
Summary

Mammalian IgG activates the human complement system, interfering with assays. Chicken IgG does not activate complement, offering a solution for more reliable solid-phase immunometric assays.

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Area of Science:

  • Immunology
  • Biochemistry

Background:

  • MicroELISA plates coated with mammalian immunoglobulin G (IgG) can activate the human complement system.
  • Complement activation by IgG can interfere with the accuracy of solid-phase immunometric assays.
  • Species and IgG subclass variations influence complement activation potential.

Purpose of the Study:

  • To investigate the complement-activating properties of mammalian and avian IgG.
  • To determine if avian IgG can be used to mitigate complement activation in immunoassays.

Main Methods:

  • Comparative analysis of IgG from different species (mammalian vs. avian).
  • Assessment of human complement system activation by coated MicroELISA plates.

Main Results:

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  • Mammalian IgG activates the human complement system.
  • Avian IgG, specifically chicken IgG, does not activate the human complement system.
  • Chicken IgG demonstrated no complement activation in the study.

    • Conclusions:

    • Chicken IgG is a suitable alternative to mammalian IgG for coating MicroELISA plates.
    • Utilizing chicken IgG can significantly reduce interference from complement activation in solid-phase immunometric assays.
    • This finding enhances the reliability and accuracy of diagnostic immunoassays.