Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cytokine expression by epidermal cell subpopulations.

H Matsue1, P D Cruz, P R Bergstresser

  • 1Department of Dermatology, UT Southwestern Medical Center, Dallas 75235.

The Journal of Investigative Dermatology
|November 11, 1992
PubMed
Summary

Epidermal cells produce cytokines. Langerhans cells are the main source of interleukin 1 beta and macrophage inflammatory protein-1 alpha mRNA in unstimulated mouse epidermal cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Survival outcomes in POD1UM-303/InterAACT-2: a phase 3 study of retifanlimab plus carboplatin-paclitaxel in first-line advanced squamous anal cancer.

Annals of oncology : official journal of the European Society for Medical Oncology·2026
Same author

ENVELOPE: enfortumab vedotin in patients with advanced small bowel adenocarcinoma refractory or intolerant to platinum-based therapy-phase II investigator-initiated trial (NCCH2412/MK015).

ESMO gastrointestinal oncology·2026
Same author

VOLTAGE-2: multicenter phase II study of nivolumab monotherapy in patients with mismatch repair-deficient resectable locally advanced rectal cancer.

ESMO gastrointestinal oncology·2026
Same author

Exploring novel therapeutic targets in small bowel adenocarcinoma: insights from claudin 18.2, nectin-4, and HER3 expression analysis.

ESMO open·2025
Same author

Effect of Whey-Derived Lactopeptide β-Lactolin on Memory in Healthy Adults: An Integrated Analysis of Data from Randomized Controlled Trials.

The journal of nutrition, health & aging·2022
Same author

Dermoscopic features of pseudoxanthoma elasticum.

Clinical and experimental dermatology·2017

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Epidermal cells (EC) are crucial for skin immunity, producing cytokines that influence local and systemic cellular functions.
  • Cytokine expression by EC subpopulations is complex and requires organized data for comprehensive understanding.
  • Existing data on EC cytokine profiles are tabulated based on cell source, activation state, and assay methods.

Purpose of the Study:

  • To investigate and identify the primary sources of specific cytokine messenger RNA (mRNA) within distinct epidermal cell subpopulations.
  • To establish a baseline understanding of cytokine production in unstimulated epidermal cells.

Main Methods:

  • Reverse transcriptase-polymerase chain reaction (RT-PCR) was utilized to detect and quantify cytokine mRNA levels.

Related Experiment Videos

  • Analysis focused on unstimulated mouse epidermal cells.

    • Main Results:

    • Langerhans cells (LC) were identified as the principal producers of mRNA for interleukin 1 beta (IL-1β).
    • Langerhans cells (LC) were also identified as the principal producers of mRNA for macrophage inflammatory protein-1 alpha (MIP-1α).
    • These findings were specific to unstimulated mouse epidermal cells.

    Conclusions:

    • Langerhans cells play a significant role in the early inflammatory response of the skin through the production of IL-1β and MIP-1α.
    • The study provides specific insights into cytokine expression patterns within epidermal subpopulations, particularly highlighting the contribution of Langerhans cells.