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Updated: Jul 25, 2026
Orthotopic Hind Limb Transplantation in the Mouse
Published on: February 12, 2016
FURTHER STUDIES ON SPECIFIC TRANSPLANTATION ANTIGENS IN ROUS SARCOMA OF MICE
Abstract:
Mice allografted with different sarcomas, induced by the Schmidt-Ruppin variant of Rous sarcoma virus (RSV-SR), showed a resistance against subsequent isografting of 9 different Rous sarcomas. Transplantation resistance could also be induced by Rous mouse tumor cells x-irradiated with 8000 r or with cell-free tumor extracts, containing no demonstrable virus. No transplantation resistance could be demonstrated after allograft pretreatment with various polyoma tumors or non-viral tumors. Allograft pretreatment with Rous tumors induced no demonstrable resistance against isografting of polyoma tumors. Inoculation of RSV-SR or Rous chicken sarcoma suspension into adult mice gave no clear cut resistance against isografting of mouse sarcomas. Neither after allografting of Rous tumors nor after virus or chicken sarcoma inoculation into adult mice could virus-neutralizing activity be demonstrated in the sera. The results demonstrate the presence of common, specific transplantation antigen(s) in different Rous sarcomas in mice and speak against an identity between the transplantation antigen(s) and viral antigen(s).
Insights
Mice developed resistance to Rous sarcoma virus (RSV-SR) tumors after initial exposure. This resistance involved specific transplantation antigens, distinct from viral antigens, highlighting tumor-specific immunity.
Area of Science:
- Oncology
- Immunology
- Virology
Background:
- Rous sarcoma virus (RSV-SR) induces sarcomas in mice.
- Tumor transplantation immunity is a complex phenomenon involving host-antigen interactions.
Purpose of the Study:
- To investigate the nature of transplantation antigens in RSV-SR induced mouse sarcomas.
- To determine if these antigens are related to viral antigens.
Main Methods:
- Mice were allografted with different RSV-SR induced sarcomas.
- Tumor resistance was assessed after pretreatment with irradiated tumor cells or cell-free extracts.
- Cross-resistance studies were performed with polyoma and non-viral tumors.
- Virus-neutralizing activity in sera was measured.
Main Results:
- Allografting with different Rous sarcomas induced specific transplantation resistance against subsequent Rous sarcoma isografts.
- Irradiated Rous tumor cells and cell-free extracts also induced resistance.
- No resistance was observed with polyoma or non-viral tumors, nor cross-resistance between Rous and polyoma tumors.
- Virus inoculation or Rous tumor allografting did not induce demonstrable virus-neutralizing antibodies.
Conclusions:
- Different Rous sarcomas in mice share common, specific transplantation antigens.
- These transplantation antigens are distinct from viral antigens associated with RSV-SR.
- The findings suggest a tumor-specific immune response rather than a direct anti-viral response dictates transplantation resistance.
