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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
08:51

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

Published on: November 4, 2016

CHARACTERIZATION OF STAPHYLOCOCCI ISOLATED FROM RAW MILK

R ZEMELMAN, L LONGERI

    Applied Microbiology
    |March 1, 1965
    PubMed

    Insights

    Beta-hemolysin production, identified using the CAMP test, is the most reliable indicator for detecting pathogenic Staphylococcus strains in bovine raw milk. This method effectively distinguishes harmful bacteria from non-pathogenic ones.

    Area of Science:

    • Veterinary Microbiology
    • Food Safety
    • Bacteriology

    Background:

    • Staphylococci are common bacteria found in bovine raw milk.
    • Determining the pathogenicity of these strains is crucial for public health and animal welfare.
    • Traditional tests for pathogenicity can yield false positives.

    Purpose of the Study:

    • To evaluate the pathogenicity of staphylococci isolated from bovine raw milk.
    • To compare the effectiveness of different tests in identifying pathogenic strains.
    • To assess the utility of beta-hemolysin production as an indicator of pathogenicity.

    Main Methods:

    • Isolation and characterization of 775 staphylococcal strains from 798 bovine raw milk samples.
    • Coagulase testing using rabbit plasma.
    • Evaluation of chromogenesis, mannitol fermentation, gelatin liquefaction, and beta-hemolysin production (CAMP test).

    Main Results:

    • Of 404 coagulase-positive strains, 76.5% produced beta-hemolysin.
    • Coagulase-negative strains did not produce beta-hemolysin.
    • Beta-hemolysin production was strongly correlated with pathogenic Staphylococcus aureus strains.

    Conclusions:

    • Beta-hemolysin production, detected via the CAMP test, is a highly specific and sensitive marker for pathogenic staphylococci in bovine raw milk.
    • The CAMP test is a valuable, rapid, and reliable tool for routine bacteriological work in identifying animal staphylococci.
    • This method minimizes the risk of false positives, enhancing diagnostic accuracy.
    Keywords:
    COAGULASEEXPERIMENTAL LAB STUDYFERMENTATIONFOOD CONTAMINATIONGELATINHEMOLYSINSMANNITOLMILKPHARMACOLOGYPIGMENTSSTAPHYLOCOCCUS

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