Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Amiloride-sensitive lithium reabsorption in rats: a micropuncture study.

R Fransen1, W H Boer, P Boer

  • 1Utrecht University Hospital, Department of Nephrology, The Netherlands.

The Journal of Pharmacology and Experimental Therapeutics
|November 1, 1992
PubMed
Summary

In sodium-restricted rats, lithium is reabsorbed in the collecting tubule, a process blocked by amiloride. This finding impacts how lithium clearance estimates kidney function.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sports-related injuries and illnesses amongst adolescent athletes in an urban sports medicine practice setting: a one-year prospective study.

South African journal of sports medicine·2025
Same author

The truth on current peritoneal dialysis: state of the art.

The Netherlands journal of medicine·2017
Same author

Acute reactions to polysulfone/polyethersulfone dialysers: literature review and management.

The Netherlands journal of medicine·2017
Same author

Application and feasibility of systemic lupus erythematosus reproductive health care quality indicators at a public urban rheumatology clinic.

Lupus·2014
Same author

Management of encapsulating peritoneal sclerosis: a guideline on optimal and uniform treatment.

The Netherlands journal of medicine·2011
Same author

Patients' representations of their end-stage renal disease: relation with mortality.

Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association·2009

Area of Science:

  • Nephrology
  • Renal Physiology

Background:

  • Lithium reabsorption in the kidney is crucial for maintaining lithium homeostasis.
  • Previous studies suggested lithium reabsorption occurs in the collecting tubule, potentially inhibited by amiloride, especially under sodium restriction.

Purpose of the Study:

  • To investigate lithium reabsorption beyond the early distal tubule in sodium-restricted rats.
  • To determine the effect of amiloride on this reabsorption process.
  • To assess the impact of amiloride on overall lithium and fluid handling in the nephron.

Main Methods:

  • Cortical micropuncture studies in sodium-restricted rats.
  • Infusion of amiloride to assess its effects on lithium and fluid reabsorption.
  • Measurement of lithium, sodium, and water reabsorption in different nephron segments.

Related Experiment Videos

Main Results:

  • Sodium-restricted rats reabsorbed 10.5% of filtered lithium beyond the early distal tubule.
  • Amiloride infusion abolished this distal lithium reabsorption.
  • Amiloride did not affect proximal tubule reabsorption but tended to increase loop segment lithium reabsorption (P = .06).
  • Amiloride decreased lithium delivery to the early distal tubule (26.1% vs. 31.4% in controls, P < .05).
  • Amiloride increased urine flow and decreased urine osmolality.

Conclusions:

  • Lithium reabsorption occurs beyond the early distal tubule in sodium-restricted rats.
  • Amiloride effectively prevents this distal lithium reabsorption.
  • Lithium clearance measurements may underestimate end-proximal delivery in sodium-restricted rats.
  • Amiloride's potential to increase upstream lithium reabsorption requires further investigation and may affect clearance data interpretation.