INHIBITION BY EXOGENOUS INTERFERON OF REPLICATION OF POLIOVIRUS RIBONUCLEIC ACID IN CHICK BRAIN

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Published on: October 11, 2013

J S YOUNGNER, M E KELLY

    Journal of Bacteriology
    |August 1, 1965
    PubMed

    Insights

    Exogenous interferon completely inhibited poliovirus ribonucleic acid (RNA) replication in chick brains. Even a small dose of interferon, administered remotely, protected the target organ from viral replication.

    Area of Science:

    • Virology
    • Immunology

    Background:

    • Poliovirus ribonucleic acid (RNA) replication can be studied in young chicks via intracerebral inoculation.
    • Young chicks are not susceptible to intact poliovirus, making them a suitable model for RNA replication studies.

    Purpose of the Study:

    • To investigate the inhibitory effect of exogenous interferon on poliovirus RNA replication in vivo.
    • To establish a model for studying the protective effects of passively transferred interferon on target organs.

    Main Methods:

    • Infectious poliovirus RNA was intracerebrally inoculated into 2-day-old chicks.
    • Exogenous interferon was administered intraperitoneally prior to RNA inoculation.
    • Viral replication was assessed, and interferon efficacy was measured in units via plaque-reduction assays.

    Main Results:

    • Single-cycle poliovirus replication peaked at approximately 12 hours post-inoculation.
    • Intraperitoneal interferon injection completely inhibited poliovirus RNA replication.
    • As little as 500 units of interferon suppressed viral replication when injected 24 hours before infectious RNA.

    Conclusions:

    • Exogenous interferon effectively inhibits poliovirus RNA replication in the chick brain.
    • This model demonstrates that interferon injected at a distance can protect target organs.
    • Passive transfer of interferon offers a potential strategy for organ protection against viral infections.

    Keywords:
    BRAINCHICK EMBRYOEXPERIMENTAL LAB STUDYIMMUNOLOGYINFLUENZA VIRUSESINTERFERONMODELS, THEORETICALPHARMACOLOGYPOLIOVIRUSPOULTRYRNA, VIRALVIRUS CULTIVATION

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