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EFFECTS OF LACTIC ACID-FORMING BACTERIA ON VIBRIO COMMA INOCULATED INTO INTESTINAL SEGMENTS OF RABBITS
Abstract:
Hattori, H. (Sankyo Co., Ltd., Tokyo, Japan), H. Misawa, I. Igarashi, and Y. Sugiya. Effects of lactic acid-forming bacteria on Vibrio comma inoculated into intestinal segments of rabbits. J. Bacteriol. 90:541-545. 1965.-Mixed inocula of Vibrio comma KC-4 and various lactic acid-forming bacteria were injected into the intestinal segments of rabbits (De and Chatterje, 1953) to observe the effects of the latter agents in altering the changes produced by strain KC-4. The animals were sacrificed 10 and 20 hr after inoculation. The inoculated intestinal segments were first examined grossly, and the amount of exudate in the segments, if any, was measured, after which the tissues were subjected to pathological examination. When KC-4 cells together with spore-bearing lactic acid-forming bacilli, strain P-22, or Lactobacillus casei were introduced, the intestinal segments showed few or no macroscopic and microscopic changes, and no accumulation of exudate. With mixed inoculation with lactic acid bacteria such as L. bulgaricus, L. acidophilus, Streptococcus lactis, and S. faecalis, changes were produced by strain KC-4. Macroscopically, no difference was discernible between the changes caused by mixed inoculation and those produced by single inoculation of KC-4. Upon pathological examination, however, it was seen that changes resulting from mixed inoculation were slightly less severe than those produced by inoculation with strain KC-4 only.
Insights
Certain lactic acid bacteria, like Lactobacillus casei, protect rabbit intestines from Vibrio cholerae (Vibrio comma) effects. Other lactic acid bacteria showed limited protective benefits against this pathogen.
Area of Science:
- Microbiology
- Gastroenterology
- Immunology
Background:
- Vibrio cholerae (Vibrio comma) causes intestinal infections.
- Lactic acid-forming bacteria are known for their potential health benefits.
Purpose of the Study:
- To investigate the protective effects of various lactic acid-forming bacteria against Vibrio cholerae infection in rabbit intestinal segments.
Main Methods:
- Rabbit intestinal segments were inoculated with Vibrio cholerae (KC-4) alone or in combination with different lactic acid-forming bacteria.
- Segments were examined macroscopically and microscopically 10 and 20 hours post-inoculation.
- Exudate accumulation and pathological changes were assessed.
Main Results:
- Co-inoculation with spore-bearing lactic acid bacilli (P-22) or Lactobacillus casei significantly reduced or eliminated macroscopic and microscopic intestinal changes caused by Vibrio cholerae.
- Co-inoculation with other lactic acid bacteria (L. bulgaricus, L. acidophilus, Streptococcus lactis, S. faecalis) did not prevent macroscopic changes but resulted in slightly less severe pathological alterations compared to Vibrio cholerae alone.
Conclusions:
- Specific strains of lactic acid-forming bacteria, particularly Lactobacillus casei, demonstrate significant protective potential against Vibrio cholerae-induced intestinal damage.
- The findings suggest a strain-dependent efficacy of lactic acid bacteria in mitigating bacterial enteropathogen effects.
