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Effect of erythromycin on L-threonine transport in rabbit jejunum in vitro
H Navarro1, M P Arruebo, V Sorribas
1Departamento de Biomedicina, Fisiologiá, Facultad de Veterinaria, Universidad de Zaragoza, Spain.
Abstract:
Several antibiotics characterized by different molecular structures are known to affect some intestinal activities. Some of them have been described as inhibitors of the intestinal sugar and amino acid transport with different mechanisms. Erythromycin (EM) is a macrolide antibiotic acting as a motilin agonist and thus stimulating the gastrointestinal motor activity. Since several substances which increase the motor activity of the gastrointestinal tract may produce effects on the intestinal absorption of nutrients, the present study has been carried out to determine whether erythromycin affects the L-threonine intestinal absorption. The results obtained indicate that erythromycin diminishes the L-threonine intestinal transport, probably at the mucosal border level. Two groups of experiments carried out, with Na(+)-deprived medium and ouabain-enriched medium, might indicate that erythromycin action could be due to either a direct or an indirect action on the Na(+)-dependent L-threonine transport located in the brush border.
Insights
Erythromycin (EM), an antibiotic, was found to reduce L-threonine absorption in the intestine. This effect may involve the sodium-dependent transport system located in the brush border of intestinal cells.
Area of Science:
- Pharmacology
- Gastroenterology
- Molecular Biology
Background:
- Antibiotics can impact intestinal functions, including nutrient transport.
- Erythromycin (EM), a macrolide antibiotic, stimulates gastrointestinal motility by acting as a motilin agonist.
Purpose of the Study:
- To investigate the effect of erythromycin on L-threonine intestinal absorption.
- To elucidate the potential mechanism underlying erythromycin's influence on nutrient transport.
Main Methods:
- In vitro experiments assessing L-threonine transport in intestinal models.
- Utilizing sodium-deprived and ouabain-enriched media to probe transport mechanisms.
Main Results:
- Erythromycin significantly reduced L-threonine intestinal transport.
- Evidence suggests the effect occurs at the brush border level.
- Findings point towards a potential interaction with the Na(+)-dependent transport system.
Conclusions:
- Erythromycin administration impairs L-threonine intestinal absorption.
- The mechanism may involve direct or indirect modulation of the Na(+)-dependent L-threonine transporter in the brush border.