Synergistic effects of intratumor administration of cis-diamminedichloroplatinum(II) combined with local hyperthermia...

Area of Science:

• Oncology
• Cancer Research
• Pharmacology

Background:

• Cis-diamminedichloroplatinum (II) (DDP) is a platinum-based chemotherapy agent.
• Local hyperthermia (LHT) can enhance the efficacy of certain cancer treatments.
• B16 melanoma is a common model for studying melanoma progression and treatment.

Purpose of the Study:

• To investigate the synergistic effect of LHT and intratumor injection (i.t.) of DDP on B16 melanoma.
• To evaluate the impact of combined therapy on tumor growth and survival in a rodent model.
• To assess potential side effects such as renal injury and metastasis.

Main Methods:

• Rodent model with implanted B16 melanoma tumors.
• Administration of DDP via intratumor injection (i.t.) or intraperitoneal (i.p.) injection.
• Application of local hyperthermia (LHT) at 42.5°C for 30 minutes post-DDP administration.
• Monitoring tumor growth ratio (TGR) and mean survival time.

Main Results:

• DDP (3 mg/kg i.t.) with LHT resulted in a tumor growth ratio (TGR) of 1.1 in experiment I and 0.5 in experiment II.
• Statistically significant differences in TGR were observed compared to control groups (P < 0.01).
• Mean survival time was significantly improved with combined therapy (42.1 days in exp I, 50.2 days in exp II; P < 0.001 in exp II).

Conclusions:

• Concomitant intratumor chemotherapy with DDP and local hyperthermia significantly enhances anticancer effects.
• This combined approach improves prognosis in B16 melanoma-bearing rodents.
• The treatment did not induce severe renal injury or promote hematogenic metastasis.