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[Interaction of mRNA of the p53 protein gene with ribosomal RNA]

O V Matveeva, P M Chumakov

    Molekuliarnaia Biologiia
    |July 1, 1992
    PubMed

    Insights

    Eukaryotic ribosomal RNAs (rRNAs), specifically 18S and 28S rRNA, can bind to messenger RNAs (mRNAs). This specific binding suggests complementary sequences exist between rRNA and mRNA fragments.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Biochemistry

    Background:

    • Ribosomal RNAs (rRNAs) are key components of ribosomes, essential for protein synthesis.
    • Messenger RNAs (mRNAs) carry genetic code from DNA to ribosomes for translation.
    • The precise interactions between rRNA and mRNA are crucial for gene expression regulation.

    Purpose of the Study:

    • To investigate the specific binding interactions between eukaryotic ribosomal RNAs (rRNAs) and messenger RNAs (mRNAs).
    • To determine if rRNA fragments can form complexes with mRNA sequences.
    • To explore the potential for sequence complementarity between rRNA and mRNA.

    Main Methods:

    • Blot hybridization techniques were employed to detect binding between DNA fragments of eukaryotic 18S and 28S rRNAs and mRNA.
    • In vitro transcribed mRNA from the mouse p53 gene was used as a model mRNA.
    • Intermolecular complex formation between rRNAs and various gene mRNAs was analyzed.

    Main Results:

    • Specific binding was observed between DNA fragments of eukaryotic 18S and 28S rRNAs and mRNA.
    • Both 18S and 28S rRNAs formed intermolecular complexes with mRNAs from several genes.
    • No binding was detected between these rRNAs and the antisense RNAs of the same genes.
    • Experimental data suggest the presence of complementary fragments within 18S and 28S rRNAs and mRNA sequences.

    Conclusions:

    • Eukaryotic 18S and 28S rRNAs contain sequences complementary to specific fragments within mRNA.
    • These complementary regions facilitate the formation of stable rRNA-mRNA intermolecular complexes.
    • This interaction mechanism may play a role in gene regulation or mRNA processing.

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