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Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection

A N Glazer1, H S Rye

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Nature
|October 29, 1992
PubMed

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Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...