Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Selective disruption of energy flow from phycobilisomes to Photosystem I.

Photosynthesis research·2013
Same author

Cryptomonad biliproteins - an evolutionary perspective.

Photosynthesis research·2013
Same author

Cryptomonad biliproteins: Bilin types and locations.

Photosynthesis research·2013
Same author

Phycobilisomes: macromolecular structure and energy flow dynamics.

Biophysical journal·2009
Same author

Roger Yate Stanier, 1916-1982: a transcendent journey.

International microbiology : the official journal of the Spanish Society for Microbiology·2002
Same author

Biosynthesis of a fluorescent cyanobacterial C-phycocyanin holo-alpha subunit in a heterologous host.

Proceedings of the National Academy of Sciences of the United States of America·2001

Related Experiment Video

Updated: Jul 8, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection.

A N Glazer1, H S Rye

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Nature
|October 29, 1992
PubMed
Summary

New fluorescent labels were created using polycationic ligands and double-stranded DNA. These multichromophore labels offer novel applications for advanced fluorescence assays.

More Related Videos

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer
04:55

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Published on: July 25, 2025

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast
09:19

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast

Published on: March 6, 2026

Related Experiment Videos

Last Updated: Jul 8, 2026

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications
10:12

Quantitative Detection of DNA-Protein Crosslinks and Their Post-Translational Modifications

Published on: April 21, 2023

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer
04:55

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Published on: July 25, 2025

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast
09:19

Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast

Published on: March 6, 2026

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Background:

  • Fluorescence assays are crucial for biological and chemical analysis.
  • Development of novel labeling agents is essential for enhancing assay sensitivity and multiplexing capabilities.

Purpose of the Study:

  • To introduce a new class of multichromophore labels for fluorescence assays.
  • To explore the formation and properties of fluorescent intercalation complexes.

Main Methods:

  • Synthesis and characterization of polycationic ligands.
  • Complexation of ligands with double-stranded DNA.
  • Spectroscopic analysis of the resulting fluorescent intercalation complexes.

Main Results:

  • Formation of stable fluorescent intercalation complexes between polycationic ligands and double-stranded DNA.
  • Demonstration of multichromophore properties within these complexes.
  • Potential for tunable fluorescence emission based on ligand design.

Conclusions:

  • Polycationic ligands form novel fluorescent intercalation complexes with DNA.
  • These complexes represent a new class of multichromophore labels.
  • The findings open avenues for advanced fluorescence-based detection methods.