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Related Experiment Videos

Fully enzymatic inulin determination in small volume samples without deproteinization.

H F Kuehnle1, K von Dahl, F H Schmidt

  • 1Forschungslaboratorien der Boehringer-Mannheim GmbH, BRD.

Nephron
|January 1, 1992
PubMed
Summary

A new enzymatic method accurately measures inulin in serum or plasma without deproteinization. This sensitive assay simplifies inulin determination using small sample volumes for clinical research.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry
  • Analytical Chemistry

Background:

  • Inulin is a polysaccharide used to assess kidney function.
  • Accurate determination of inulin in biological samples is crucial for clinical diagnostics.
  • Existing methods for inulin measurement can be time-consuming or require sample pre-treatment.

Purpose of the Study:

  • To develop and validate a fully enzymatic method for quantifying inulin in serum or plasma.
  • To establish a sensitive, specific, and accurate assay that minimizes sample preparation.
  • To demonstrate the method's utility in analyzing inulin blood levels in vivo.

Main Methods:

  • Enzymatic hydrolysis of inulin to fructose using inulinase.
  • Simultaneous oxidation of native glucose using glucose oxidase and hydrogen peroxide (H2O2).

Related Experiment Videos

  • Fructose determination as the basis for inulin quantification.

    • Main Results:

    • The described method provides a fully enzymatic determination of inulin without deproteinization.
    • The assay exhibits high specificity, sensitivity, and accuracy.
    • The procedure is time-saving and requires only small sample volumes.
    • The method's applicability was confirmed by analyzing human and rabbit blood inulin levels post-intravenous Inutest administration.

    Conclusions:

    • A novel, efficient enzymatic method for inulin determination in serum and plasma has been established.
    • This assay offers a significant advancement for routine clinical and research applications.
    • The method's simplicity and accuracy facilitate reliable inulin blood level monitoring.