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HLA and MLC typing in patients with Hodgkin's disease
Insights
Human Leukocyte Antigen (HLA) A1 antigen is increased in Caucasian Hodgkin's disease patients. No significant HLA-D locus antigen deviations were observed in this patient group.
Area of Science:
- Immunogenetics
- Oncology
- Human Leukocyte Antigen (HLA) system
Background:
- Hodgkin's disease (HD) is a lymphoma with complex etiology.
- The Human Leukocyte Antigen (HLA) system plays a crucial role in immune regulation and disease susceptibility.
- Previous studies suggest potential associations between specific HLA antigens and various cancers, including HD.
Purpose of the Study:
- To investigate the frequencies of HLA A and B locus antigens in Caucasian patients with Hodgkin's disease.
- To compare HLA antigen profiles between HD patients and healthy controls.
- To examine HLA-D locus antigen specificities in a subset of HD patients.
Main Methods:
- HLA typing for 27 A and B locus antigens was performed on 137 Caucasian HD patients.
- Antigen frequencies were compared between the total patient group, a prospective cohort, and long-term survivors (>5 years).
- Mixed Lymphocyte Culture (MLC) typing for HLA-D locus was conducted on 51 unselected patients using homozygous typing cells.
Main Results:
- A significant decrease in HLA-AW33 frequency was observed in HD patients (0.0%) compared to controls (5.9%, p < 0.01).
- HLA-A1 antigen frequency was significantly increased in the entire HD patient group (35.8%) versus North American Caucasians (25.4%, p < 0.02).
- No significant deviations in HLA A, B, or D locus antigen frequencies were found concerning patient sex, histology, age, or survival duration.
Conclusions:
- The study identifies a potential association between increased HLA-A1 antigen and Hodgkin's disease in Caucasian populations.
- The decreased frequency of HLA-AW33 warrants further investigation.
- No significant associations were found for HLA-D locus antigens or other HLA A/B antigens with clinical parameters in this cohort.
Abstract:
HLA typing for 27 A and B locus antigens was performed in 137 Caucasian patients with Hodgkin's disease. A and B locus antigen frequencies were compared for the entire group of patients, for 51 patients studied in a prospective manner and for 62 patients surviving with Hodgkin's disease for more than 5 years. MLC typing (HLA-D) was performed in 51 unselected Caucasian patients using 6 differend HLA-D homozygous test cells which define 3 HLA-D specificity groups (DW2, DW3, and DW4). HLA-AW33 was found in 0.0% of patients and in 5.9% of random Caucasian blood donors at the Sloan--Kettering Institute (X2 equals 7.04, p less than 0.01). HLA-A1 was found to be increased to 35.8% in the entire patient group (N = 137) as compared to 25.4% in 855 random North American Caucasians (X2 = 5.97, p less than 0.02). There was no significant deviation of any A or B locus antigen in relation to sex, histology, age of patient, or duration of survival (newly diagnosed vs 5-year survivors). No significant deviation for either of the 3 common HLA-D determinants (DW2, DW3, or DW4) was observed.