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Local radiolabeling of the 68 kDa neurofilament protein in rat sciatic nerves
J R Sotelo1, C R Benech, A Kun
1División Biofisica, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.
Neuroscience Letters
|September 14, 1992
Abstract:
Rat sciatic nerve segments, 4.5 cm distal to the dorsal root ganglion (DRG), were incubated in vivo with [35S]methionine. Fluorography of 2-D polyacrylamide gels of the nerve proteins demonstrated the labeling of the 68-kDa neurofilament protein, which was identified by immunoblotting. This experimental design excludes the dorsal root ganglion as the source of the radiolabeled neurofilament protein and suggests that this neuron-specific protein may be synthesized in axons.