Related Experiment Video

Updated: Aug 17, 2026

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures
08:30

One-day Workflow Scheme for Bacterial Pathogen Detection and Antimicrobial Resistance Testing from Blood Cultures

Published on: July 9, 2012

[Sensitivity of multiresistant bacterial strains to imipenem (Tienam)]

J Ruczkowska1, I Dolna

  • 1Katedry i Zakładu Mikrobiologii Lekarskiej AM, Wrocławiu.

Przeglad Lekarski
|January 1, 1992
PubMed

Insights

Imipenem demonstrates high effectiveness against Gram-negative rods resistant to multiple cephalosporins. Over 90% of these multi-drug resistant strains remained susceptible to imipenem in vitro.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Gram-negative rods are a significant cause of hospital-acquired infections.
  • Increasing resistance to cephalosporins and aminoglycosides poses a therapeutic challenge.
  • Multidrug-resistant Gram-negative bacteria require effective alternative treatment options.

Purpose of the Study:

  • To evaluate the in vitro susceptibility of clinical Gram-negative rod strains to imipenem.
  • To assess imipenem's activity against strains resistant to multiple cephalosporins and aminoglycosides.

Main Methods:

  • Testing susceptibility of 213 clinical Gram-negative rod strains to imipenem.
  • Comparing imipenem susceptibility with ceftazidime activity.
  • Analyzing co-resistance patterns with aminoglycosides.

Main Results:

  • 93.9% of strains resistant to six cephalosporins were susceptible to imipenem.
  • Only 6.1% of these strains exhibited resistance to imipenem.
  • Imipenem showed high activity against multidrug-resistant Gram-negative rods, with 64.3% being imipenem-sensitive and ceftazidime-resistant.

Conclusions:

  • Imipenem is a highly active antibiotic against a broad spectrum of multidrug-resistant Gram-negative rods.
  • Imipenem represents a valuable therapeutic option for infections caused by cephalosporin-resistant Gram-negative bacteria.

More Related Videos

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method12:03

Antimicrobial Synergy Testing by the Inkjet Printer-assisted Automated Checkerboard Array and the Manual Time-kill Method

Published on: April 18, 2019

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Related Concept Videos

Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...