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Related Experiment Videos

Granulocyte oxidative activity after thermal injury.

W G Cioffi1, D G Burleson, B S Jordan

  • 1U.S. Army Institute of Surgical Research, Fort Sam Houston, TX 78234-5012.

Surgery
|November 11, 1992
PubMed
Summary

Burn patients show increased granulocyte activity. Their white blood cells (granulocytes) exhibit higher baseline and stimulated cytosolic peroxidase activity, indicating in vivo activation after thermal injury.

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Area of Science:

  • Immunology
  • Burn Medicine
  • Cellular Biology

Background:

  • Granulocyte dysfunction is a known complication following thermal injury.
  • Previous studies have indicated alterations in granulocyte function post-burn.
  • This study investigates serial changes in granulocyte cytosolic peroxidase activity in burn patients.

Purpose of the Study:

  • To serially assess granulocyte cytosolic peroxidase activity in patients with thermal injuries.
  • To determine if granulocytes from burn patients exhibit altered activity compared to healthy controls.
  • To investigate potential in vivo activation of granulocytes post-burn.

Main Methods:

  • Purified granulocytes were isolated from peripheral blood of 23 thermally injured patients.
  • Dichlorofluorescin diacetate was used to measure baseline and phorbol myristate acetate-stimulated fluorescence via flow cytometry.

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  • Granulocytes from unburned subjects served as daily controls.

    • Main Results:

    • Unstimulated granulocytes from burn patients showed significantly higher baseline activity (22.9%) compared to controls (15.4%; p < 0.05).
    • Stimulated granulocytes from patients exhibited 114% of the fluorescence intensity observed in control granulocytes (p < 0.05).
    • These findings suggest increased cytosolic oxidase activity in granulocytes from thermally injured individuals.

    Conclusions:

    • Granulocytes from thermally injured patients demonstrate elevated baseline cytosolic oxidase activity.
    • This suggests in vivo activation of granulocytes following thermal injury.
    • Burn patients' granulocytes also show enhanced activity upon in vitro stimulation.