Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Potential-dependent alpha-latrotoxin interaction with black lipid membranes.

A N Chanturia1, V K Lishko

  • 1Department of Neurochemistry, A.V. Palladin Biochemistry Institute, Kiev, Ukraine.

Toxicon : Official Journal of the International Society on Toxinology
|September 1, 1992
PubMed
Summary

Positive electrical potentials applied to lipid membranes promote alpha-latrotoxin (LTX) channel formation. A two-step model explains LTX binding and insertion, driven by the electric field, influencing membrane interactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibrinogen counteracts the antiadhesive effect of fibrin-bound plasminogen by preventing its activation by adherent U937 monocytic cells.

Journal of thrombosis and haemostasis : JTH·2012
Same author

Plasminogen on the surfaces of fibrin clots prevents adhesion of leukocytes and platelets.

Journal of thrombosis and haemostasis : JTH·2010
Same author

The alternatively spliced alpha(E)C domain of human fibrinogen-420 is a novel ligand for leukocyte integrins alpha(M)beta(2) and alpha(X)beta(2).

Blood·2001
Same author

Probing the structure-function relationship of alpha-latrotoxin-formed channels with antibodies and pronase.

Toxicon : official journal of the International Society on Toxinology·1996
Same author

[Interaction of precursors of secretory proteins and components of a cell-free protein-synthesizing system with phospholipid membranes].

Ukrainskii biokhimicheskii zhurnal (1978)·1996
Same author

[Interaction of ribosomes with membranes in vitro].

Ukrainskii biokhimicheskii zhurnal (1978)·1996

Area of Science:

  • Biophysics
  • Membrane Biology
  • Neuroscience

Background:

  • Alpha-latrotoxin (LTX) is a potent neurotoxin that interacts with presynaptic membranes.
  • Understanding LTX interaction with lipid bilayers is crucial for elucidating its mechanism of action.
  • Membrane potential is a key factor influencing protein-membrane interactions.

Purpose of the Study:

  • To investigate the effect of membrane potential on alpha-latrotoxin insertion into bilayer lipid membranes (BLM).
  • To propose a model explaining the interaction between LTX and lipid bilayers under electrical influence.

Main Methods:

  • Electrophysiological measurements of channel formation in BLM upon LTX application.
  • Varying the electrical potential across the BLM (cis-positive potentials).

Related Experiment Videos

  • Comparative analysis with existing data on LTX-synaptosomal membrane interactions.

    • Main Results:

    • Positive electrical potentials applied cis to the toxin significantly stimulated channel formation in BLM.
    • A two-step model was proposed: irreversible binding followed by rapid insertion and channel formation.
    • The electric field across the BLM is suggested as the driving force for LTX insertion.

    Conclusions:

    • Membrane potential plays a critical role in modulating alpha-latrotoxin's ability to form channels in lipid bilayers.
    • The proposed two-step model provides a framework for understanding LTX-membrane electrodynamics.
    • These findings offer insights into LTX neurotoxicity and potential therapeutic strategies.