Related Experiment Videos
The 45 kDa and 27 kDa yeast's protein kinases are not immunologically related
R Szyszka1, M Tchórzewski, P Dukowski
1Department of Molecular Biology, Maria Curie-Sklodowska University, Lublin, Poland.
Acta Biochimica Polonica
|January 1, 1992
Abstract:
Two yeast casein kinase type-1 species of 45 kDa and 27 kDa (CK1) were purified to apparent homogeneity and used for investigation of their immunological affinity. Antisera against the two kinases were isolated; the antibody against the 45 kDa kinase did not react with the 27 kDa enzyme. The 27 kDa casein kinase was recognized only by its own antibody. The obtained data strongly suggest that the low molecular mass CK-1 is not a proteolytic product of the 45 kDa kinase species.