Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualization of myocardial cellular architecture using acoustic microscopy.

P A Chandraratna1, S Choudhary, J P Jones

  • 1Division of Cardiology, USC School of Medicine, Los Angeles 90033.

American Heart Journal
|November 1, 1992
PubMed
Summary

High-frequency ultrasound, or acoustic microscopy, visualizes microscopic structures like cardiac tissue at 1 micron resolution. This unstained imaging method correlates closely with light microscopy for pathology detection.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Search for Light Pseudoscalar Bosons, Pair-Produced in Higgs Boson Decays in the Four-Electron Final State in Proton-Proton Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

First Evidence for Mixing-Induced CP Violation in B_{s}^{0}→J/ψϕ(1020) Decays in pp Collisions at sqrt[s]=13  TeV.

Physical review letters·2026
Same author

Comparative outcomes with low-dose and standard-dose immune checkpoint inhibitors in microsatellite stable advanced gastric/gastroesophageal adenocarcinoma.

ESMO gastrointestinal oncology·2026
Same author

Measurement of D^{0} Meson Photoproduction in Ultraperipheral Heavy Ion Collisions.

Physical review letters·2026
Same author

Observation of tWZ Production at the CMS Experiment.

Physical review letters·2026
Same author

First Exclusive Reconstruction of the B^{*+}, B^{*0}, and B_{s}^{*0} Mesons and Precise Measurement of Their Masses.

Physical review letters·2026

Area of Science:

  • Biomedical Engineering
  • Pathology
  • Microscopy

Background:

  • Ultrasound transducer resolution is frequency-dependent, improving with higher frequencies.
  • High-frequency ultrasound (600-1000 MHz) enables microscopic visualization.
  • Acoustic microscopy offers a potential high-resolution imaging modality.

Purpose of the Study:

  • To evaluate the efficacy of scanning acoustic microscopy for visualizing myocardial biopsy specimens.
  • To compare the diagnostic capabilities of acoustic microscopy with traditional light microscopy.
  • To assess the resolution and detail provided by acoustic microscopy in cardiac tissue.

Main Methods:

  • Unstained, deparaffinized 5-micron myocardial biopsy sections from 10 patients were imaged using a 1000 MHz Olympus UH3 scanning acoustic microscope.

Related Experiment Videos

  • Acoustic microscopy images were compared with subsequent hematoxylin and eosin and PTAH-stained light microscopy images of serial sections.
  • The study focused on imaging myocytes, myofibrils, interstitial tissue, and intramural vessels.

    • Main Results:

    • Acoustic microscopy accurately imaged cardiac cellular structures, including myocytes, myofibrils, and interstitial tissue.
    • Pathologic findings such as cell fallout, fibrosis, and lymphocytic infiltration were identifiable.
    • Intramural vessels, endothelial cell nuclei, and media were clearly visualized, showing close correlation with light microscopy findings.
    • The technique achieved a resolution comparable to light microscopy without the need for specimen staining.

    Conclusions:

    • Scanning acoustic microscopy provides high-resolution visualization of cardiac cellular detail, comparable to light microscopy.
    • This technique can identify microscopic cardiac pathologies in unstained specimens.
    • Acoustic microscopy presents a valuable, staining-free alternative for analyzing myocardial biopsy samples.