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Mutations of the Ki-ras oncogene in endometrial carcinoma
D Ignar-Trowbridge1, J I Risinger, G A Dent
1Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709.
Objective:
The purpose of this study was to assess the extent of involvement of the ras oncogene in endometrial carcinoma.
Study Design:
Genomic deoxyribonucleic acid from 30 samples of endometrial carcinoma was examined for point mutations in codons 12, 13, and 61 from the Ha-ras, Ki-ras, and N-ras genes by means of the polymerase chain reaction, slot-blotting, and deoxyribonucleic acid sequencing procedures.
Results:
An apparent somatic mutation of Ki-ras codon 12 in one of 10 paraffin-embedded tumors was confirmed by deoxyribonucleic acid sequence analysis. Two of 20 frozen endometrial carcinoma specimens were also shown to contain a point mutation in Ki-ras codon 12. No correlation between ras mutation and a number of histologic or clinical parameters was observed.
Conclusions:
These data suggest a potential role for Ki-ras codon 12 mutations in the development of some (10%) endometrial cancers.