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Suction effusion fluid from skin and constituent analysis: new candidate for interstitial fluid
S Kayashima1, T Arai, M Kikuchi
1Third Department of Internal Medicine, National Defense Medical College, Saitama, Japan.
The American Journal of Physiology
|November 1, 1992
Summary
Suction effusion fluid (SEF) mirrors serum concentrations for small molecules like glucose, offering a new method for monitoring blood glucose levels in real-time. This fluid, collected from skin, shows potential for non-invasive glucose monitoring.
Area of Science:
- Biomedical Engineering
- Dermatology
- Metabolic Research
Background:
- Transcutaneous fluid analysis offers a minimally invasive approach to monitoring physiological parameters.
- Understanding the composition of suction effusion fluid (SEF) is crucial for its potential diagnostic applications.
Purpose of the Study:
- To analyze the biochemical composition of suction effusion fluid (SEF).
- To assess the feasibility of monitoring glucose concentration in SEF during intravenous glucose loading.
- To determine the relationship between SEF glucose and blood glucose levels.
Main Methods:
- SEF was collected from rabbits using suction from corneal layer-stripped skin.
- Fluid constituents, including glucose, creatinine, urea nitrogen, and proteins, were analyzed and compared to serum.
- SEF glucose concentration was monitored every 10 minutes during intravenous glucose loading.
Main Results:
- SEF demonstrated similar concentrations to serum for low-molecular-weight substances like glucose, creatinine, and urea nitrogen.
- SEF protein concentration was approximately one-fourth of serum protein concentration, with minimal detection of proteins > 100 kDa.
- SEF glucose levels successfully tracked blood glucose levels with a 10-minute delay.
Conclusions:
- SEF composition is comparable to interstitial fluid and effusate from subcutaneous vessels.
- SEF is a viable medium for monitoring glucose concentrations, reflecting blood glucose dynamics with a slight delay.
- This technique holds promise for non-invasive glucose monitoring in clinical settings.
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