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Cloning and expression of the aspartate carbamoyltransferase gene from Treponema denticola
K Ishihara1, M Ishihara, I Takazoe
1Department of Microbiology, Tokyo Dental College, Chiba, Japan.
Abstract:
Treponema denticola seems to play a central role in the etiology of human periodontal disease. We have cloned an antigenic protein-coding sequence from T. denticola ATCC 33520. The protein-coding region was found to be a 3-kbp HindIII-HindIII fragment. The open reading frame consists of 1,426 bp and codes for a protein with an M(r) of 54,919. The deduced amino acid sequence showed 33.8% homology with that of the aspartate carbamoyltransferase of Escherichia coli. The gene products showed aspartate carbamoyltransferase activity.
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