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Related Experiment Videos

Screening for microorganisms producing D-malate from maleate.

M J van der Werf1, W J van den Tweel, S Hartmans

  • 1Department of Food Science, Wageningen Agricultural University, The Netherlands.

Applied and Environmental Microbiology
|September 1, 1992
PubMed
Summary

Researchers screened over 300 microorganisms for maleate conversion to D-malate using maleate hydratase. Many strains efficiently produced high-purity D-malate, with Pseudomonas pseudoalcaligenes showing exceptional enantiomeric purity.

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Area of Science:

  • Biotechnology
  • Enzymology
  • Microbial Metabolism

Background:

  • Maleate is a substrate that can be converted into malate isomers.
  • Enzymatic conversion of maleate to D-malate is of interest for biotechnological applications.
  • Maleate hydratase is a key enzyme in this transformation.

Purpose of the Study:

  • To screen a diverse range of microorganisms for maleate hydratase activity.
  • To identify microbial strains capable of producing enantiomerically pure D-malate.
  • To characterize the D-malate production by a selected strain.

Main Methods:

  • Screening of over 300 microbial strains for maleate conversion.
  • Incubation of permeabilized cells with maleate.
  • Analysis of fumarate and malate accumulation to determine enzyme activity and product enantiomeric purity.

Related Experiment Videos

  • Detailed study of Pseudomonas pseudoalcaligenes NCIMB 9867.

    • Main Results:

    • 128 microbial strains demonstrated the ability to convert maleate to D-malate with >97% enantiomeric purity.
    • Pseudomonas pseudoalcaligenes NCIMB 9867 degraded maleate completely, producing D-malate with >99.97% enantiomeric purity.
    • Fumarate accumulation served as an indicator for maleate transformation.

    Conclusions:

    • A significant number of microorganisms possess maleate hydratase activity for D-malate production.
    • Pseudomonas pseudoalcaligenes NCIMB 9867 is a highly efficient producer of enantiomerically pure D-malate.
    • This study identifies promising biocatalysts for D-malate synthesis.