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Isolation and characteristics of bacteriophages from staphylococci of chicken origin

A Shimizu

    American Journal of Veterinary Research
    |September 1, 1977
    PubMed

    Insights

    Most chicken staphylococci strains are phage carriers. New bacteriophages (CH phages) effectively type chicken staphylococci, including strains untypeable by international phage sets.

    Area of Science:

    • Microbiology
    • Bacteriology
    • Virology

    Background:

    • Coagulase-positive staphylococci are significant in poultry.
    • Lysogeny, the integration of phage DNA into bacterial genomes, is common in staphylococci.
    • Accurate typing of staphylococcal strains is crucial for epidemiological studies.

    Purpose of the Study:

    • To investigate lysogenicity in chicken coagulase-positive staphylococci.
    • To isolate and characterize novel bacteriophages from chicken staphylococci.
    • To evaluate the efficacy of these new bacteriophages for typing chicken staphylococcal strains.

    Main Methods:

    • Lysogenicity testing using mitomycin C induction.
    • Isolation of bacteriophages from lysogenic chicken staphylococci.
    • Phage typing of chicken staphylococcal strains using international and newly isolated (CH) phages.

    Main Results:

    • High prevalence of lysogeny (95.5%) was observed in tested chicken staphylococci strains.
    • Newly isolated CH phages demonstrated high efficacy in typing chicken staphylococci (82.8% typeable).
    • CH phages were effective in typing strains resistant to international phage sets, showing high specificity for chicken staphylococci.

    Conclusions:

    • Chicken coagulase-positive staphylococci exhibit high rates of lysogeny.
    • The 16 newly isolated CH phages are valuable tools for the specific and efficient typing of chicken staphylococci.
    • These CH phages offer significant advantages over international phage sets for characterizing staphylococcal isolates from poultry.

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