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Adenine phosphoribosyl transferase polymorphism in baboons
J L VandeBerg1, M J Aivaliotis
1Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78228-0147.
Biochemical Genetics
|August 1, 1992
Summary
Two adenine phosphoribosyl transferase (APRT) variants were identified in baboons, showing clear genetic inheritance. This enzyme
Area of Science:
- Genetics and Molecular Biology
- Primate Biology
- Biochemistry
Background:
- Adenine phosphoribosyl transferase (APRT) is a crucial enzyme in purine metabolism.
- Genetic variations in APRT can impact cellular function and disease susceptibility.
- Baboons serve as valuable models for studying human genetic diseases.
Purpose of the Study:
- To identify and characterize allelic isozymes of adenine phosphoribosyl transferase (APRT) in baboons.
- To determine the inheritance pattern of APRT variants.
- To assess the utility of baboon APRT as a genetic marker.
Main Methods:
- Starch gel electrophoresis was used to detect APRT isozymes in baboon hemolysates.
- Family studies were conducted to verify inheritance patterns.
- Gene frequencies were analyzed across five baboon subspecies.
Main Results:
- Two allelic isozymes of APRT were identified in baboons.
- Autosomal codominant inheritance was confirmed through extensive family data.
- Significant differences in APRT gene frequencies were observed among baboon subspecies.
- Erythrocyte APRT activity is high, suitable for marker use.
Conclusions:
- The genetic basis of APRT in baboons has been elucidated.
- APRT serves as a reliable genetic marker in baboons.
- This enzyme can be a sensitive indicator for chimerism in bone marrow transplantation research.