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Related Experiment Videos

beta-lactamases in Shigella.

H Schumacher1, M Nir, B Mansa

  • 1Department of Hospital Infections and Antibiotics, Statens Seruminstitut, Copenhagen, Denmark.

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
|October 1, 1992
PubMed
Summary

This study found ampicillin resistance in nearly half of Shigella strains, particularly Sh. flexneri. However, all strains remained susceptible to newer antibiotics like cefotaxime, indicating potential treatment options.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Shigella species are significant causes of bacterial dysentery worldwide.
  • Antimicrobial resistance in Shigella poses a growing public health threat.
  • Beta-lactamase production is a primary mechanism of resistance to beta-lactam antibiotics.

Purpose of the Study:

  • To investigate the prevalence of ampicillin resistance in Shigella isolates.
  • To characterize the types of beta-lactamases produced by these strains.
  • To assess susceptibility to newer antimicrobial agents.

Main Methods:

  • Sixty Shigella strains were collected and tested for antimicrobial susceptibility.
  • Phenotypic methods were used to detect beta-lactamase production.

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  • Molecular characterization of beta-lactamases was performed.

    • Main Results:

    • Ampicillin resistance was observed in 47% of Shigella isolates, with higher rates in Sh. flexneri compared to Sh. sonnei.
    • Beta-lactamases identified included TEM-1, OXA-1, and a chromosomally mediated cephalosporinase.
    • Sh. flexneri strains predominantly produced OXA-1-like beta-lactamases.
    • All tested strains remained susceptible to cefotaxime, mecillinam, and ciprofloxacin.

    Conclusions:

    • Ampicillin resistance is prevalent in Shigella, necessitating careful antibiotic selection.
    • The identified beta-lactamases are common resistance mechanisms.
    • Susceptibility to third-generation cephalosporins and fluoroquinolones offers viable treatment alternatives for Shigella infections.