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Physical Isolation of Endospores from Environmental Samples by Targeted Lysis of Vegetative Cells
Published on: January 21, 2016
Effects of some mechanical factors on the endospores of Bacillus subtilis
Abstract:
Knaysi, Georges (Cornell University, Ithaca, N. Y.) and Harold R. Curran. Effects of some mechanical factors on the endospores of Bacillus subtilis. J. Bacteriol. 82:691-694. 1961.-Phase contrast microscopy was used to study incipient spore germination and early growth under conditions involving agitation with a platinum wire loop, sonic oscillation, and freezing and thawing. Treatment resulted in change in optical properties and destruction of a certain proportion of the spores. Up to one-tenth of the spores was destroyed by loop agitation. Sonic oscillation had little apparent effect upon incipient germination (darkening) of the spores, but subsequent development involving growth was stimulated in a considerable proportion. The effect of freezing and thawing upon incipient germination is discussed.
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