[Study of the abnormal plasma proteins after burn injury]

In our previous study at least 4 abnormal bands were found in burned sera when analyzed by SDS-PAGE. In this paper further exploration of the characteristics of these abnormalities of burned sera were presented. By way of western blotting, 12 kinds of antisera against different human proteins as detecting antibodies were used. Results showed that bands developed by anti-haptoglobin antiserum corresponded well with the abnormal bands in SDS-PAGE. If the burned serum was separated by ion-exchange chromatography and its collected eluates containing protein peaks were tested for the immunosuppressive activities on normal murine spleen lymphocytes, the immunosuppressive eluates were usually found in the 6th protein peak. When these immunosuppressive eluates were further analyzed by SDS-PAGE and Western blotting, bands stained with anti-haptoglobin antiserum still coincided with the abnormal bands found in SDS-PAGE of burned serum. Result of this study suggests that one of the immunosuppressive factors found in burned serum may be a haptoglobin-like substance.