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Tetrapeptide QDPR is a minimal immunodominant epitope within the preS2 domain of hepatitis B virus
I Sominskaya1, V Bichko, P Pushko
1Latvian Medical Academy, Riga.
Immunology Letters
|July 1, 1992
Abstract:
A hepatitis B virus preS2 deletion library with the preS2 sequence fused to the coat protein of the RNA phage fr (fr CP) as a carrier has been constructed and used for the approximate localization of epitope recognized by a panel of murine monoclonal anti-preS2 antibodies. DNA copies of putative preS2 epitopes were synthesized and cloned within the fr CP gene. Tetrapeptide Gln-Asp-Pro-Arg (QDPR) corresponding to the preS (132-135) sequence was found to be the minimal sufficient recognition site for one of the monoclonal antibodies, S26. The closely related tetrapeptide EDPR did not mimic the epitope activity of QDPR.