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TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene
1Department of Bioscience and Biotechnology, Drexel University, Philadelphia, Pennsylvania 19104.
Journal of Bacteriology
|December 1, 1992
Abstract:
We report the existence of a sixth replication arrest site, TerF, that is located within the coding sequences of the rcsC gene, a negative regulator of capsule biosynthesis. The TerF site is oriented to allow transcription of the rcsC gene but prevent DNA replication in the terminus-to-origin direction. Our results demonstrate that the TerF site is functional in both chromosomal and plasmid environments and that the stability of the Tus-TerF protein-DNA complex more closely resembles the plasmid R6K Ter sites than the chromosomal TerB site.