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Related Experiment Videos

Apoptosis in metanephric development.

C Koseki1, D Herzlinger, Q al-Awqati

  • 1Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

The Journal of Cell Biology
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

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Metanephric development involves mesenchymal-to-epithelial transition, but surrounding cells undergo programmed cell death (apoptosis). Inducers like embryonic spinal cord prevent apoptosis, requiring two steps: apoptosis rescue and differentiation induction.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Metanephric development requires mesenchymal cells to form epithelial nephron structures.
  • Induction involves extracellular matrix proteins and factors from the ureteric bud or heterologous cells.
  • Programmed cell death (apoptosis) occurs in cells surrounding newly formed epithelia.

Purpose of the Study:

  • To investigate the role of apoptosis in metanephric development.
  • To identify mechanisms by which inducers prevent apoptosis and promote differentiation.
  • To elucidate the signaling pathways involved in mesenchymal-to-epithelial transition.

Main Methods:

  • In vitro and in vivo induction of embryonic mesenchyme.
  • Morphological assessment of apoptosis.

Related Experiment Videos

  • Biochemical analysis of DNA degradation.
  • Treatment with apoptosis inhibitors (actinomycin-D, cycloheximide) and calcium buffering.
  • Testing effects of protein kinase C modulators (phorbol esters, staurosporine) and EGF.

    • Main Results:

    • Uninduced mesenchyme exhibits DNA degradation, a marker of apoptosis.
    • Apoptosis is prevented by actinomycin-D, cycloheximide, calcium buffering, and heterologous inducers.
    • Protein kinase C activation mimics inducer effects; inhibition blocks them.
    • EGF prevents DNA degradation but not differentiation.

    Conclusions:

    • Metanephric mesenchyme is programmed for apoptosis during development.
    • Mesenchymal-to-epithelial conversion requires two distinct steps: rescue from apoptosis and induction of differentiation.
    • Protein kinase C signaling is involved in preventing apoptosis during this process.