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Gluconate metabolism of Pasteurellapestis

R P MORTLOCK

    Journal of Bacteriology
    |July 1, 1962
    PubMed

    Insights

    Pasteurella pestis utilizes gluconate through two key metabolic pathways: the 6-phosphogluconate dehydrogenase-transketolase pathway and the Entner-Doudoroff pathway. This study identified essential enzymes involved in Pasteurella pestis gluconate metabolism.

    Area of Science:

    • Microbiology
    • Biochemistry
    • Bacteriology

    Background:

    • Pasteurella pestis is a virulent bacterium responsible for plague.
    • Understanding bacterial metabolism is crucial for developing effective treatments and control strategies.
    • Gluconate metabolism plays a role in the energy production and virulence of various microorganisms.

    Purpose of the Study:

    • To investigate the gluconate metabolic pathways in a virulent strain of Pasteurella pestis.
    • To identify the key enzymes involved in gluconate degradation by Pasteurella pestis.
    • To determine the specific routes of 6-phosphogluconate metabolism.

    Main Methods:

    • Preparation of cell-free extracts from Pasteurella pestis.
    • Enzymatic assays to detect the presence of specific metabolic enzymes.
    • Analysis of the degradation products of 6-phosphogluconate.

    Main Results:

    • Evidence for gluconokinase, 6-phosphogluconate dehydrogenase, transketolase, and 2-keto-3-deoxy-6-phosphogluconate dehydrase in cell-free extracts.
    • 6-phosphogluconate was metabolized via both the 6-phosphogluconate dehydrogenase-transketolase and Entner-Doudoroff pathways.
    • No active glucose-6-phosphate dehydrogenase was detected.

    Conclusions:

    • Pasteurella pestis employs distinct enzymatic pathways for gluconate metabolism.
    • The identified enzymes and pathways provide insights into the bacterium's metabolic capabilities.
    • Further research can explore the implications of these pathways for Pasteurella pestis virulence and survival.
    Keywords:
    GLUCONATES/metabolismPASTEURELLA PESTIS/metabolism

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