Comparison of growth and pectolytic enzyme production by Bacillus polymyxa

Nagel, Charles W. (University of California, Davis) and Reese H. Vaughn. Comparison of growth and pectolytic enzyme production by Bacillus polymyxa. J. Bacteriol. 83:1-5. 1962.-Studies were made of pectolytic enzyme production by Bacillus polymyxa during growth. It was found that elaboration of enzyme occurred during logarithmic growth and ceased when the stationary phase was reached. The specific activity of the extra-cellular enzyme remained relatively constant until lysis occurred. The increased specific activity of the intracellular pectolytic enzyme may be explained if one assumes that the rate of secretion of the intracellular enzyme is dependent upon the concentration of the extracellular enzyme. The concentration of the intracellular pectolytic enzyme dropped markedly at the end of the logarithmic growth phase; the enzyme was released into the medium during the stationary growth phase and subsequent lysis of the cells. It was shown that the intra- and extracellular enzymes were similar or identical in that both were calcium-dependent pectic acid transeliminases.