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ACR1, a yeast ATF/CREB repressor.

A C Vincent1, K Struhl

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.

Molecular and Cellular Biology
|December 1, 1992
PubMed
Summary
This summary is machine-generated.

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Researchers identified ACR1, a new yeast gene encoding a transcriptional repressor. This discovery reveals a family of ATF/CREB proteins in yeast that can activate or repress transcription.

Area of Science:

  • Molecular Biology
  • Genetics
  • Yeast Biology

Background:

  • Mammalian ATF/CREB transcription factors regulate genes via cyclic AMP and viral oncogenes, binding specific DNA sequences (TGACGTCA) through a bZIP domain.
  • In yeast (Saccharomyces cerevisiae), ATF/CREB-like sequences can either activate or repress transcription depending on promoter context.

Purpose of the Study:

  • To identify yeast genes involved in regulating ATF/CREB-mediated transcriptional repression.
  • To characterize the function and DNA-binding properties of newly identified regulatory factors.

Main Methods:

  • Isolation of yeast mutations that alleviate repression at ATF/CREB sites.
  • Gene cloning and sequencing to identify the mutated gene (ACR1).
  • Analysis of ACR1 gene function through deletion and examination of transcriptional activity.

Related Experiment Videos

  • Biochemical assays to assess DNA-binding activities in yeast extracts.

    • Main Results:

    • A novel yeast gene, ACR1, was identified, encoding a transcriptional repressor of the ATF/CREB family.
    • ACR1 possesses a bZIP domain crucial for homodimerization and specific binding to ATF/CREB DNA sites.
    • ACR1 shows structural similarity to mammalian CREB/CREM and differs from yeast activators GCN4/YAP1.
    • Deletion of ACR1 leads to increased transcription at ATF/CREB sites, independent of GCN4 or YAP1.
    • Yeast extracts from acr1 deletion strains exhibit elevated ATF/CREB-like DNA-binding activities.

    Conclusions:

    • Saccharomyces cerevisiae possesses a family of ATF/CREB proteins that function as both transcriptional repressors and activators.
    • ACR1 is a key repressor within this family, playing a significant role in regulating gene expression via ATF/CREB sites.
    • The findings suggest a complex regulatory network of ATF/CREB-like factors in yeast transcription.