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Rapid quality control of 99Tcm-sestamibi
R M Reilly1, M So, J Polihronis
1Division of Nuclear Medicine, Toronto Hospital, Ontario, Canada.
Nuclear Medicine Communications
|September 1, 1992
Summary
A new Sep-Pak chromatography method offers a faster way to determine the radiochemical purity of technetium-99m-sestamibi (99Tcm-sestamibi). While slightly overestimating purity, this rapid technique is practical for clinical use.
Area of Science:
- Nuclear Medicine
- Radiopharmaceutical Chemistry
Background:
- The standard thin-layer chromatographic (TLC) method for assessing 99Tcm-sestamibi radiochemical purity is time-consuming.
- Accurate purity determination is crucial for effective diagnostic imaging.
Purpose of the Study:
- To evaluate a reversed-phase chromatography (Sep-Pak) technique as a faster alternative to TLC for measuring 99Tcm-sestamibi radiochemical purity.
- To compare the accuracy and practicality of the Sep-Pak method against the established TLC procedure.
Main Methods:
- Comparison of the Sep-Pak technique with the manufacturer-recommended TLC method.
- Quantification of free 99Tcm-pertechnetate levels in 99Tcm-sestamibi preparations.
Main Results:
- The Sep-Pak technique accurately determined free 99Tcm-pertechnetate levels.
- A minor overestimation of radiochemical purity (approx. 3%) was observed with the Sep-Pak method due to binding of reduced-hydrolyzed 99Tcm.
- This overestimation was deemed clinically insignificant.
Conclusions:
- The Sep-Pak technique provides a rapid and practical method for determining the radiochemical purity of 99Tcm-sestamibi.
- Despite a slight overestimation, the Sep-Pak method is a viable alternative to the slower TLC technique for routine quality control.