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Updated: Aug 12, 2026
Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
Substrate preference of metalloproteinases secreted by ts-110 Moloney murine sarcoma virus-transformed normal rat
H Z Zhang1, M Scanlon, J L Murray
1Department of Tumor Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Abstract:
In this study, the two forms of affinity-purified transformation-associated proteins (TAPs) (68 and 64 kD) were shown to have different substrate preferences. For the 68-kD TAP, the order of substrate preference was collagen types I, III, and V; fibronectin; gelatin; and collagen IV. For the 64-kD TAP, the order of substrate preference was collagen I, III, and V and gelatin. The 64-kD TAP did not cleave collagen IV and fibronectin. We also found a 71-kD metalloproteinase in the concentrated purified TAPs that reacted only weakly with a TAP monoclonal antibody and showed this substrate preference: collagen I, III, and V; gelatin; and collagen IV. Whether this 71-kD TAP is a new member of the rat metalloproteinase family will be investigated.
