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04:36
Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
Yeast cell adhesion on oligopeptide modified surfaces.
S S Dhadwar1, T Bemman, W A Anderson
1Department of Chemical Engineering, University of Waterloo, 200 University Avenue, Waterloo, Ontario, Canada N2L 3G1.
Biotechnology Advances
|September 23, 2003
Summary
Self-assembling oligopeptides like EAK 16 II can alter cell adhesion on surfaces. Yeast cell adhesion decreased on plastic but slightly increased on glass, suggesting hydrophobic interactions are key.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Cell Biology
Background:
- Self-assembling oligopeptides offer novel bioengineering applications.
- Modifying substrate surface properties is crucial for controlling cell adhesion.
- EAK 16 II is a specific oligopeptide with potential for surface functionalization.
Purpose of the Study:
- To investigate the effect of EAK 16 II oligopeptide on cell-supporting substrate surface properties.
- To analyze the impact of EAK 16 II surface modification on yeast cell adhesion (Candida utilis).
- To correlate surface property changes with observed cell adhesion and morphology.
Main Methods:
- Thermodynamic measurements (liquid contact angle, surface free energy) to characterize surface properties.
- Atomic Force Microscopy (AFM) to observe surface morphology and cell adhesion.
- Quantitative analysis of yeast cell adhesion on modified and unmodified hydrophobic (plastic) and hydrophilic (glass) substrates.
Main Results:
- A critical concentration of 0.1 mg/ml EAK 16 II was determined for complete surface modification.
- Yeast cell adhesion was significantly reduced on plastic but slightly enhanced on glass substrates coated with EAK 16 II.
- AFM imaging revealed cell morphology changes and suggested differences in water retention based on substrate type.
Conclusions:
- EAK 16 II effectively modifies substrate surface properties, influencing yeast cell adhesion.
- Hydrophobic interactions play a significant role in yeast cell adhesion to surfaces.
- Surface roughness and oligopeptide coating can modulate cell adhesion and potentially cell water retention.
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