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Related Experiment Videos

Yeast cell adhesion on oligopeptide modified surfaces.

S S Dhadwar1, T Bemman, W A Anderson

  • 1Department of Chemical Engineering, University of Waterloo, 200 University Avenue, Waterloo, Ontario, Canada N2L 3G1.

Biotechnology Advances
|September 23, 2003
PubMed
Summary

Self-assembling oligopeptides like EAK 16 II can alter cell adhesion on surfaces. Yeast cell adhesion decreased on plastic but slightly increased on glass, suggesting hydrophobic interactions are key.

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Cell Biology

Background:

  • Self-assembling oligopeptides offer novel bioengineering applications.
  • Modifying substrate surface properties is crucial for controlling cell adhesion.
  • EAK 16 II is a specific oligopeptide with potential for surface functionalization.

Purpose of the Study:

  • To investigate the effect of EAK 16 II oligopeptide on cell-supporting substrate surface properties.
  • To analyze the impact of EAK 16 II surface modification on yeast cell adhesion (Candida utilis).
  • To correlate surface property changes with observed cell adhesion and morphology.

Main Methods:

  • Thermodynamic measurements (liquid contact angle, surface free energy) to characterize surface properties.

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  • Atomic Force Microscopy (AFM) to observe surface morphology and cell adhesion.
  • Quantitative analysis of yeast cell adhesion on modified and unmodified hydrophobic (plastic) and hydrophilic (glass) substrates.

    • Main Results:

    • A critical concentration of 0.1 mg/ml EAK 16 II was determined for complete surface modification.
    • Yeast cell adhesion was significantly reduced on plastic but slightly enhanced on glass substrates coated with EAK 16 II.
    • AFM imaging revealed cell morphology changes and suggested differences in water retention based on substrate type.

    Conclusions:

    • EAK 16 II effectively modifies substrate surface properties, influencing yeast cell adhesion.
    • Hydrophobic interactions play a significant role in yeast cell adhesion to surfaces.
    • Surface roughness and oligopeptide coating can modulate cell adhesion and potentially cell water retention.