Recombinant autoantigens for diagnosis and therapy of autoimmune diseases

The characteristic of autoimmune diseases resides in the existence of T- and B-cell autoreactivity when directed against self-proteins. Thus autoantibodies are considered to be of diagnostic relevance. The advent of recombinant protein expression technology has made it possible to produce economically high-quality autoantigens for use in immunoassays for detecting autoantibodies in patients' sera. The intention of this short review is to give the reader a brief description of autoantigen production methodologies with their advantages, disadvantages and the limitations of these techniques in the diagnostics of autoimmune diseases. Further possible usage of recombinant autoantigens as tools in investigation and therapy of autoimmune diseases is discussed.