Related Experiment Video
Updated: Aug 31, 2026

Endotracheal Intubation of Rabbits Using a Polypropylene Guide Catheter
Published on: November 13, 2017
Prepared endotracheal tubes: are they a potential source for pathogenic microorganisms?
Nivez F Rasic1, Robert M Friesen, Bruce Anderson
1Department of Anesthesia, St. Boniface General Hospital, Winnipeg, Manitoba, Canada.
Unlabelled:
Prepared endotracheal tubes (PETTs) are frequently used for unanticipated difficult intubation, but their storage time is highly variable and institution-dependent. We sought to determine first, if open, unused PETTs are a potential source of pathogenic microorganisms, and second, if PETTs can provide a medium for bacterial survival after deliberate contamination. A stylet was inserted into a 7-mm ETT, and this system was ethylene oxide sterilized. The PETTs were placed in 20 different locations and sampled 8 times in a 4-wk period. Growth was determined after 48-h incubation, and the microorganism was identified. In Phase 2, the PETT (n = 40) was swabbed with a fresh suspension of H. influenzae, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecium, or a negative control. Nonvirulent bacteria were cultured from 13 of 160 (8.1%) samples and from 15 of 320 (4.7%) samples in Phases 1 and 2, respectively. No PETT grew the same bacteria more than once. In Phase 2, after 24 h, only E. faecium was recovered. Based on this study, the pathogenic potential of PETTs is very small, and they can be safely used for up to 1 mo. This practice could translate to significant cost reduction for operating room budgets.
Implications:
Prepared endotracheal tubes (PETTs) are back-up airway equipment to be used in the case of a difficult intubation. A short PETT shelf life because of unknown safe storage time results in significant budget costs. This blinded, controlled study examined the pathogenic potential of PETTs in the operating room environment.
Insights
Prepared endotracheal tubes (PETTs) can be safely stored for up to one month. This study found minimal pathogenic potential in PETTs, suggesting extended use can reduce operating room budget costs.
Area of Science:
- Medical Devices
- Microbiology
- Anesthesiology
Background:
- Prepared endotracheal tubes (PETTs) are critical for managing difficult intubations.
- Current storage times for PETTs are variable and institution-dependent, leading to significant costs.
- The potential for microbial contamination and survival on PETTs requires investigation.
Purpose of the Study:
- To assess if unused PETTs harbor pathogenic microorganisms.
- To determine if PETTs support bacterial survival after contamination.
- To establish a safe storage duration for PETTs to reduce healthcare costs.
Main Methods:
- Phase 1: PETTs were placed in various operating room locations and sampled over 4 weeks.
- Phase 2: PETTs were deliberately inoculated with common respiratory pathogens (H. influenzae, P. aeruginosa, S. aureus, E. faecium) and controls.
- Microbial growth and identification were assessed after incubation; bacterial survival was monitored over 24 hours.
Main Results:
- Nonvirulent bacteria were found in 8.1% of Phase 1 samples and 4.7% of Phase 2 samples.
- No single PETT showed growth of the same bacteria more than once.
- After 24 hours in Phase 2, only Enterococcus faecium was recovered, indicating limited bacterial survival.
Conclusions:
- The pathogenic potential of prepared endotracheal tubes is minimal.
- PETTs can be safely used for up to one month, supporting extended shelf life.
- Implementing a one-month storage policy for PETTs can lead to substantial cost savings for operating rooms.
Related Concept Videos
Endotracheal Intubation I: Procedure
The ET tube comprises various components, including a standard adaptor to attach a bag-valve-mask (BVM) or ventilator, a cuff, a pilot balloon, and radiopaque markings along its length to measure the insertion distance. The tube sizes...
Microbiota of the Respiratory Tract
Endotracheal Tube Extubation
Procedure
Extubation removes the endotracheal tube (ETT) from the patient on mechanical ventilation. It requires a well-coordinated, multidisciplinary approach involving physicians, nurses, respiratory therapists, and other healthcare professionals.
Endotracheal Intubation II: Nursing Management
1. Nursing Care of Patients Before Intubation
Before the endotracheal intubation procedure, nurses play an essential role in ensuring the process goes smoothly. The nurses must be familiar with intubation...
Tracheostomy: Procedure and Tubes
Tracheostomy tubes can be made of semiflexible plastic (polyurethane or silicone), rigid plastic, or metal, and they come in...
Oxygen Delivering System III: Tracheostomy and T-piece
Tracheostomy
A tracheostomy is a surgically created opening (stoma) in the anterior part of the trachea. It is used to establish a patient airway, bypass an upper airway obstruction, simplify the removal of secretions, permit long-term...
