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Serum amyloid P component binding to Shiga toxin 2 requires both a subunit and B pentamer
Paola Marcato1, Kathleen Vander Helm, George L Mulvey
1Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada.
Infection and Immunity
|September 23, 2003
Abstract:
Solid-phase binding, competitive binding, and cytotoxicity neutralization assays indicate that the B pentamer and A subunit both contribute to human serum amyloid P (HuSAP) component binding to Stx2. A polyvalent globotriaosyl-ceramide receptor analog, Daisy, did not competitively inhibit HuSAP binding, implying that the two ligands bind to different Stx2 domains.